BIOLOGY, Class 11

Class 11 : Biology (In English) – Lesson 12: Respiration in Plants

EXPLANATION & SUMMARY



🌱✨ Introduction
🧠 Respiration is a vital catabolic process in which food molecules (mainly glucose) are broken down to release energy for cellular activities.
🌿 Unlike photosynthesis, which stores energy, respiration releases energy in the form of ATP ⚑ β€” the energy currency of cells.


πŸ’‘ Concept:
➑️ Photosynthesis: Anabolic (builds food)
➑️ Respiration: Catabolic (breaks food)
🧬 Equation:
C₆H₁₂O₆ + 6 Oβ‚‚ β†’ 6 COβ‚‚ + 6 Hβ‚‚O + Energy (β‰ˆ 686 kcal)


🌍 This process occurs in all living cells β€” including plants β€” continuously, both day and night.



🌾 Importance of Respiration
πŸƒ Provides ATP for growth, repair, and absorption
🌸 Maintains metabolic balance
πŸ§ͺ Breaks complex compounds into simpler ones
⚑ Supports biosynthesis, transport, active absorption
✏️ Note: Though plants photosynthesize, they also respire continuously to meet energy demands.

🧬 Types of Respiration


🟒 1. Aerobic Respiration


Occurs in presence of oxygen
πŸ’¨ End products: COβ‚‚ + Hβ‚‚O + Energy
🧠 Complete oxidation of glucose
πŸ”΄ 2. Anaerobic Respiration
Occurs without oxygen
πŸ’¨ End products: Ethanol + COβ‚‚ + Energy (in plants)
⚑ Less energy released (2 ATP per glucose)
πŸ’‘ Concept:
🌾 Yeast undergoes alcoholic fermentation, forming ethanol (used in brewing).
🌱 Plant roots in waterlogged soils may temporarily switch to anaerobic mode.

🌸 Steps of Aerobic Respiration
Occurs in multiple steps β€” each in a specific site of the cell:
Step Process Site Key Products
1️⃣ Glycolysis Cytoplasm 2 ATP, 2 NADH
2️⃣ Pyruvate Oxidation Mitochondrial matrix 2 Acetyl-CoA, 2 NADH
3️⃣ Krebs Cycle Mitochondrial matrix 6 NADH, 2 FADHβ‚‚, 2 ATP
4️⃣ Electron Transport Chain (ETC) Inner mitochondrial membrane ~34 ATP
Total yield β‰ˆ 38 ATP per glucose (in theory).

🌿 Step-by-Step Explanation


πŸ§ͺ Step 1: Glycolysis
🧠 Meaning β€œsplitting of sugar”
πŸ“ Site: Cytoplasm
πŸ”¬ Occurs in both aerobic & anaerobic respiration
πŸŒ€ Steps:
1️⃣ Glucose (6C) phosphorylated β†’ Glucose-6-phosphate
2️⃣ Converted β†’ Fructose-6-phosphate
3️⃣ β†’ Fructose 1,6-bisphosphate β†’ splits into 3C units
4️⃣ Forms 2 molecules of pyruvate
⚑ Products:
2 Pyruvate
2 ATP (net)
2 NADH
πŸ’‘ Concept: Universal pathway present in all living cells.

πŸ”₯ Step 2: Oxidative Decarboxylation of Pyruvate
πŸ“ Site: Mitochondrial matrix
🎯 Pyruvate (3C) β†’ Acetyl-CoA (2C) + COβ‚‚
πŸ§ͺ Enzyme: Pyruvate dehydrogenase
🧠 NAD⁺ reduced to NADH
✏️ Note: This links glycolysis and Krebs cycle.

🌿 Step 3: Krebs Cycle (Citric Acid Cycle)


🧬 Discovered by Hans Krebs
πŸ“ Site: Mitochondrial matrix
🎯 Acetyl-CoA + Oxaloacetate (4C) β†’ Citrate (6C) β†’ undergoes series of reactions β†’ regenerates oxaloacetate
🧠 Products per glucose:
6 NADH
2 FADHβ‚‚
2 ATP
4 COβ‚‚
πŸ’‘ Concept: Central metabolic hub β€” provides intermediates for biosynthesis.

⚑ Step 4: Electron Transport Chain (ETC)


πŸ“ Site: Inner mitochondrial membrane
🧬 NADH & FADHβ‚‚ donate electrons β†’ pass through complexes (I–IV)
πŸ’§ Oxygen = final electron acceptor β†’ forms Hβ‚‚O
🧠 Chemiosmotic Theory (Mitchell): Proton gradient across membrane drives ATP synthesis through ATP synthase.
⚑ ATP Yield: ~34 ATP
πŸ’‘ Total Aerobic Gain: β‰ˆ 38 ATP/glucose

πŸƒ Anaerobic Respiration


🧠 Occurs when Oβ‚‚ absent
πŸ“ Site: Cytoplasm
🌾 Common in yeast, germinating seeds, waterlogged roots
Pathway:
Glucose β†’ Pyruvate (via glycolysis) β†’ Ethanol + COβ‚‚ + ATP
⚑ Net energy: 2 ATP
✏️ Note: Less efficient, but essential under hypoxic conditions.

πŸ§ͺ Fermentation in Plants


🌱 In yeast: Pyruvate β†’ Ethanol + COβ‚‚
🧫 Enzymes: Pyruvate decarboxylase, Alcohol dehydrogenase
🍺 Used in brewing, baking
πŸ’‘ Concept: Fermentation regenerates NAD⁺ to continue glycolysis.

🌾 Amphibolic Pathway
🧠 Respiration serves as catabolic + anabolic process
➑️ Provides intermediates for amino acid, lipid, nucleic acid synthesis
βš™οΈ Example: Acetyl-CoA β†’ fatty acids; Ξ±-ketoglutarate β†’ amino acids
πŸ’‘ Amphibolic = dual role of degradation & biosynthesis.

🌿 Respiratory Quotient (RQ)
πŸ§ͺ RQ = COβ‚‚ produced / Oβ‚‚ consumed
Substrate RQ Value
Carbohydrates 1
Fats <1 Proteins <1 Organic acids >1
πŸ’‘ Indicates substrate being respired and metabolic balance.
✏️ Example:
Glucose β†’ RQ = 1
Fat β†’ RQ = 0.7
Succinate β†’ RQ > 1

🌱 Respiratory Substrates
1️⃣ Carbohydrates: primary
2️⃣ Fats: yield more energy
3️⃣ Proteins: used under starvation
⚑ All eventually enter Krebs cycle as intermediates.

🌸 Energy Yield Summary
Step ATP Yield
Glycolysis 2 ATP + 2 NADH
Pyruvate Oxidation 2 NADH
Krebs Cycle 2 ATP + 6 NADH + 2 FADHβ‚‚
ETC ~34 ATP
Total β‰ˆ 38 ATP
🧠 Actual yield may be lower (~30–32) due to transport losses.

🌍 Factors Affecting Respiration
1️⃣ Temperature: increases rate up to optimum
2️⃣ Oxygen: required for aerobic phase
3️⃣ Substrate type: carbohydrate vs fat
4️⃣ Tissue type: active meristems > storage tissues
5️⃣ Age: younger tissues respire more
πŸ’‘ Concept: Respiration adapts to plant needs and environment.

🌿 Respiration vs Combustion
Feature Respiration Combustion
Nature Enzymatic Non-enzymatic
Control Stepwise Uncontrolled
Energy Stored in ATP Lost as heat
Site Living cells Outside cells
🧠 Respiration is controlled biological oxidation.

🌳 Significance
🌾 Provides ATP for vital activities
🧬 Supplies intermediates for biosynthesis
πŸ’¨ Maintains COβ‚‚-Oβ‚‚ balance
🧠 Integrates catabolism with anabolism
🌍 Essential for growth and survival

🌍 Why This Lesson Matters
🌿 Explains how plants extract energy from food
🧠 Links metabolism, biosynthesis, and growth
βš™οΈ Foundation for biochemistry and ecology
πŸ’¨ Highlights balance between photosynthesis and respiration

πŸ“ Quick Recap
🧠 Respiration = breakdown of food to release energy
🌱 Aerobic: Oβ‚‚ present β†’ COβ‚‚ + Hβ‚‚O + ATP (~38)
🌾 Anaerobic: no Oβ‚‚ β†’ Ethanol + COβ‚‚ + ATP (2)
βš™οΈ Steps: Glycolysis β†’ Pyruvate Oxidation β†’ Krebs β†’ ETC
πŸ’‘ Fermentation: regenerates NAD⁺
🧬 Amphibolic = catabolic + anabolic
πŸ§ͺ RQ indicates substrate used
🌍 Provides energy, intermediates, and balance

πŸ“˜ Summary
Respiration in plants is a vital energy-releasing process involving the oxidation of organic compounds. The aerobic pathway yields maximum ATP through glycolysis, Krebs cycle, and electron transport. Anaerobic respiration or fermentation operates without oxygen, releasing less energy. The process is amphibolic, integrating breakdown and biosynthesis. Factors like oxygen, temperature, and substrate influence the rate. Through respiration, plants obtain ATP and essential intermediates for various metabolic functions, maintaining cellular energy balance and sustaining life processes.

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QUESTIONS FROM TEXTBOOK



πŸ”΅ Question 1. Differentiate between:
🟒 Answer:
(a) 🌿 Respiration and Combustion
Feature Respiration Combustion
Nature Biological, enzymatic Non-biological, rapid
Energy release Step-wise, controlled Sudden, uncontrolled
Temperature Occurs at body temperature High temperature
Intermediate formation Several intermediates formed None
Example Cellular respiration Burning of fuels

(b) 🌸 Glycolysis and Krebs’ Cycle
Feature Glycolysis Krebs’ Cycle
Site Cytoplasm Mitochondrial matrix
Oxygen requirement Anaerobic phase Aerobic phase
Substrate Glucose Pyruvic acid
Products 2 Pyruvate, 2 ATP, 2 NADH COβ‚‚, NADH, FADHβ‚‚, ATP
Occurs in All cells Only aerobic cells

(c) πŸ§ͺ Aerobic Respiration and Fermentation
Feature Aerobic Respiration Fermentation
Oxygen Required Absent
End products COβ‚‚ + Hβ‚‚O Alcohol / Lactic acid
ATP yield 36–38 ATP 2 ATP
Site Mitochondria Cytoplasm
Efficiency High Low

πŸ”΅ Question 2. What are respiratory substrates? Name the most common respiratory substrate.
🟒 Answer:
🌿 Respiratory substrates are organic compounds oxidised during respiration to release energy.
πŸ’‘ Types:
Carbohydrates: e.g. glucose (most common)
Fats
Proteins
βœ”οΈ Most common: Glucose

πŸ”΅ Question 3. Give the schematic representation of glycolysis.
🟒 Answer:
🧬 Glycolysis: Conversion of glucose (6C) β†’ 2 Pyruvate (3C)
✏️ Steps description:
Glucose β†’ Glucose-6-phosphate (uses 1 ATP)
β†’ Fructose-6-phosphate
β†’ Fructose-1,6-bisphosphate (uses 1 ATP)
β†’ Splits into two 3C compounds
β†’ Glyceraldehyde-3-phosphate β†’ Pyruvic acid
βœ”οΈ Net products: 2 ATP (net gain), 2 NADH, 2 Pyruvate

πŸ”΅ Question 4. What are the main steps in aerobic respiration? Where does it take place?
🟒 Answer:
🧠 Main steps:
Glycolysis – cytoplasm
Oxidative decarboxylation – mitochondria
Krebs’ cycle – mitochondrial matrix
Electron Transport System (ETS) – inner mitochondrial membrane
βœ”οΈ End products: COβ‚‚ + Hβ‚‚O + 38 ATP

πŸ”΅ Question 5. Give the schematic representation of an overall view of Krebs’ cycle.
🟒 Answer:
🌿 Cycle description:
Acetyl CoA + Oxaloacetic acid β†’ Citric acid
Citric acid β†’ Ξ±-Ketoglutaric acid β†’ Succinic acid β†’ Malic acid β†’ Oxaloacetic acid
βœ”οΈ Products per cycle:
3 NADH, 1 FADHβ‚‚, 1 ATP (via GTP), 2 COβ‚‚
✏️ Cycle regenerates oxaloacetic acid each turn.

πŸ”΅ Question 6. Explain ETS.
🟒 Answer:
🧬 Electron Transport System (ETS):
Located in inner mitochondrial membrane
Consists of four complexes (I–IV) with electron carriers (FMN, Fe-S, cytochromes)
➑️ NADH/FADHβ‚‚ donate electrons β†’ move through complexes β†’ Oβ‚‚ is final acceptor β†’ Hβ‚‚O formed
βœ”οΈ Energy released used to pump protons β†’ ATP formed by ATP synthase = Oxidative phosphorylation

πŸ”΅ Question 7. Distinguish between:
(a) 🌸 Aerobic and Anaerobic Respiration
Feature Aerobic Anaerobic
Oxygen Present Absent
End product COβ‚‚, Hβ‚‚O Alcohol or Lactic acid
ATP yield 36–38 2
Site Mitochondria Cytoplasm

(b) πŸ§ͺ Glycolysis and Fermentation
Feature Glycolysis Fermentation
Process Breakdown of glucose to pyruvate Conversion of pyruvate to ethanol/lactate
Oxygen Independent Anaerobic
ATP 2 2
Location Cytoplasm Cytoplasm

(c) βš—οΈ Glycolysis and Citric Acid Cycle
Feature Glycolysis Citric Acid Cycle
Site Cytoplasm Mitochondria
Oβ‚‚ requirement Not required Requires Oβ‚‚
Product Pyruvate COβ‚‚, NADH, FADHβ‚‚, ATP
Type Linear pathway Cyclic pathway

πŸ”΅ Question 8. What are the assumptions made during the calculation of net gain of ATP?
🟒 Answer:
🧠 Assumptions:
Complete oxidation of glucose.
NADH from cytoplasm enters mitochondria efficiently.
ATP yield: 3 per NADH, 2 per FADHβ‚‚.
No energy loss.
βœ”οΈ Net gain: ~36 ATP per glucose molecule (theoretical).

πŸ”΅ Question 9. Discuss β€œThe respiratory pathway is an amphibolic pathway.”
🟒 Answer:
🧬 Amphibolic = both catabolic + anabolic
🌿 Catabolic: Breakdown of carbohydrates, fats, proteins β†’ energy.
🌸 Anabolic: Intermediates used for synthesis (amino acids, lipids).
βœ”οΈ Thus, respiratory pathway serves dual role.

πŸ”΅ Question 10. Define RQ. What is its value for fats?
🟒 Answer:
πŸ§ͺ Respiratory Quotient (RQ) = COβ‚‚ produced / Oβ‚‚ consumed
➑️ For fats (e.g. tripalmitin): RQ = 0.7
πŸ’‘ Because fats require more oxygen for oxidation.

πŸ”΅ Question 11. What is oxidative phosphorylation?
🟒 Answer:
⚑ Process of ATP synthesis using energy released by electron transport through ETS.
➑️ Oβ‚‚ = final electron acceptor β†’ Hβ‚‚O formed
➑️ ATP synthase utilizes proton gradient β†’ ATP
βœ”οΈ Coupled with aerobic respiration.

πŸ”΅ Question 12. What is the significance of step-wise release of energy in respiration?
🟒 Answer:
🌿 Significance:
Prevents energy loss as heat.
Allows controlled release for ATP synthesis.
Enables regulation via enzymes.
Ensures efficient energy utilisation.
βœ”οΈ Maintains metabolic balance in cell.

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OTHER IMPORTANT QUESTIONS FOR EXAMS

(CBSE MODEL QUESTIONS PAPER)

ESPECIALLY MADE FROM THIS LESSON ONLY



πŸ”΄ Question 1:
Respiration in plants is a β€”
πŸ”΄1️⃣ Catabolic process
🟒2️⃣ Anabolic process
🟑3️⃣ Both
πŸ”΅4️⃣ None
🟒 Answer: 1️⃣ Catabolic process

πŸ”΄ Question 2:
Which of the following is a common substrate for respiration?
πŸ”΄1️⃣ Protein
🟒2️⃣ Glucose
🟑3️⃣ Fat
πŸ”΅4️⃣ Organic acid
🟒 Answer: 2️⃣ Glucose

πŸ”΄ Question 3:
Where does glycolysis occur in a cell?
πŸ”΄1️⃣ Mitochondria
🟒2️⃣ Cytoplasm
🟑3️⃣ Nucleus
πŸ”΅4️⃣ Chloroplast
🟒 Answer: 2️⃣ Cytoplasm

πŸ”΄ Question 4:
The end products of aerobic respiration are β€”
πŸ”΄1️⃣ COβ‚‚ + Hβ‚‚O + ATP
🟒2️⃣ Alcohol + COβ‚‚
🟑3️⃣ Lactic acid + ATP
πŸ”΅4️⃣ Only ATP
🟒 Answer: 1️⃣ COβ‚‚ + Hβ‚‚O + ATP

πŸ”΄ Question 5:
In which organelle does Krebs cycle occur?
πŸ”΄1️⃣ Cytoplasm
🟒2️⃣ Mitochondrial matrix
🟑3️⃣ Inner membrane
πŸ”΅4️⃣ Chloroplast
🟒 Answer: 2️⃣ Mitochondrial matrix

πŸ”΄ Question 6:
Which enzyme converts pyruvate to acetyl CoA?
πŸ”΄1️⃣ Dehydrogenase
🟒2️⃣ Pyruvate dehydrogenase
🟑3️⃣ Decarboxylase
πŸ”΅4️⃣ Oxidase
🟒 Answer: 2️⃣ Pyruvate dehydrogenase

πŸ”΄ Question 7:
Number of ATP molecules produced from one glucose molecule in aerobic respiration is β€”
πŸ”΄1️⃣ 2
🟒2️⃣ 36
🟑3️⃣ 4
πŸ”΅4️⃣ 8
🟒 Answer: 2️⃣ 36

πŸ”΄ Question 8:
Which of the following processes does not require oxygen?
πŸ”΄1️⃣ Krebs cycle
🟒2️⃣ Glycolysis
🟑3️⃣ ETS
πŸ”΅4️⃣ Oxidative phosphorylation
🟒 Answer: 2️⃣ Glycolysis

πŸ”΄ Question 9:
The final electron acceptor in aerobic respiration is β€”
πŸ”΄1️⃣ NAD⁺
🟒2️⃣ Oxygen
🟑3️⃣ ATP
πŸ”΅4️⃣ Cytochrome
🟒 Answer: 2️⃣ Oxygen

πŸ”΄ Question 10:
Fermentation takes place in the β€”
πŸ”΄1️⃣ Cytoplasm
🟒2️⃣ Mitochondria
🟑3️⃣ Chloroplast
πŸ”΅4️⃣ Nucleus
🟒 Answer: 1️⃣ Cytoplasm

πŸ”΄ Question 11:
Define glycolysis.
🟒 Answer:
Stepwise breakdown of glucose (6C) into two molecules of pyruvate (3C) in cytoplasm, releasing 2 ATP and 2 NADH ⚑

πŸ”΄ Question 12:
Write two end products of alcoholic fermentation.
🟒 Answer:
Ethyl alcohol (Cβ‚‚Hβ‚…OH) 🍢
Carbon dioxide (COβ‚‚) πŸ’¨


πŸ”΄ Question 13:
What is respiration? Write its overall equation.
🟒 Answer:
Definition: Biochemical process where food molecules are broken down to release energy (ATP).
Overall Equation:
 C₆H₁₂O₆ + 6Oβ‚‚ β†’ 6COβ‚‚ + 6Hβ‚‚O + Energy (ATP) ⚑
Significance: Energy released is used for all cellular activities.

πŸ”΄ Question 14:
Describe the main stages of aerobic respiration.
🟒 Answer:
1️⃣ Glycolysis – In cytoplasm; glucose β†’ pyruvate + 2 ATP + 2 NADH.
2️⃣ Krebs Cycle – In mitochondria; acetyl CoA oxidized to COβ‚‚ + NADH + FADHβ‚‚ + ATP.
3️⃣ Electron Transport System – Inner mitochondrial membrane; NADH/FADHβ‚‚ oxidized β†’ ATP + Hβ‚‚O.

πŸ”΄ Question 15:
Explain the process of glycolysis.
🟒 Answer:
Site: Cytoplasm.
Steps:
 1️⃣ Glucose phosphorylated β†’ fructose-1,6-bisphosphate.
 2️⃣ Split into 2 triose phosphates.
 3️⃣ Oxidation β†’ 2 pyruvate, 2 ATP (net), 2 NADH.
Products: 2 Pyruvate + 2 ATP + 2 NADH.

πŸ”΄ Question 16:
Differentiate between aerobic and anaerobic respiration.
🟒 Answer:
Feature Aerobic Anaerobic
Oxygen Required Not required
End products COβ‚‚ + Hβ‚‚O Alcohol / Lactic acid
ATP yield 36 ATP 2 ATP
Site Mitochondria Cytoplasm
πŸ’‘ Aerobic is more efficient.

πŸ”΄ Question 17:
Describe the link reaction.
🟒 Answer:
Site: Mitochondrial matrix.
Reaction: Pyruvate (3C) β†’ Acetyl CoA (2C) + COβ‚‚ + NADH.
Enzyme: Pyruvate dehydrogenase.
Purpose: Connects glycolysis and Krebs cycle πŸ”—.

πŸ”΄ Question 18:
Explain the Krebs cycle.
🟒 Answer:
Site: Mitochondrial matrix.
Steps:
 1️⃣ Acetyl CoA + OAA β†’ Citrate (6C).
 2️⃣ Series of oxidation and decarboxylation.
 3️⃣ Products per turn: 3 NADH, 1 FADHβ‚‚, 1 ATP, 2 COβ‚‚.
Significance: Produces high-energy carriers.

πŸ”΄ Question 19:
What is oxidative phosphorylation?
🟒 Answer:
Definition: Formation of ATP using energy released by electron transport through ETC.
Site: Inner mitochondrial membrane.
Process: NADH & FADHβ‚‚ donate e⁻ β†’ proton gradient β†’ ATP formed by ATP synthase. ⚑
Product: 34 ATP approx.

πŸ”΄ Question 20:
Explain electron transport system (ETS).
🟒 Answer:
Location: Inner mitochondrial membrane.
Carriers: NADH β†’ FMN β†’ CoQ β†’ Cytochromes β†’ Oβ‚‚.
Function:
 1️⃣ Transfers electrons from NADH/FADHβ‚‚.
 2️⃣ Creates proton gradient.
 3️⃣ ATP formed by ATP synthase.
βœ… Oβ‚‚ is final acceptor β†’ Hβ‚‚O formed.

πŸ”΄ Question 21:
What are respiratory substrates? Name their types.
🟒 Answer:
Definition: Compounds oxidized during respiration to release energy.
Types:
 1️⃣ Carbohydrates (e.g. glucose) – main substrate.
 2️⃣ Fats – yield more energy.
 3️⃣ Proteins – used in starvation.
πŸ’‘ Preference order: Carbohydrates > Fats > Proteins.

πŸ”΄ Question 22:
Mention factors affecting rate of respiration.
🟒 Answer:
1️⃣ Temperature: Optimum ~30Β°C; low temp slows rate. 🌑️
2️⃣ Oxygen: Needed for aerobic respiration.
3️⃣ Substrate concentration: More substrate β†’ higher rate till saturation.
4️⃣ Protoplasmic condition: Active cells respire faster.

πŸ”΄ Question 23:
Explain the steps of aerobic respiration in detail.
🟒 Answer:
Step 1️⃣ β€” Glycolysis:
 Occurs in cytoplasm; glucose (6C) β†’ 2 pyruvate (3C) + 2 ATP + 2 NADH.
Step 2️⃣ β€” Link Reaction:
 In mitochondria; pyruvate β†’ acetyl CoA + COβ‚‚ + NADH.
Step 3️⃣ β€” Krebs Cycle:
 Acetyl CoA oxidized β†’ COβ‚‚ + NADH + FADHβ‚‚ + ATP.
Step 4️⃣ β€” Electron Transport System:
 Electrons from NADH/FADHβ‚‚ β†’ ETC β†’ ATP by oxidative phosphorylation; Oβ‚‚ final acceptor.
βœ… Products per glucose: 36 ATP + 6 COβ‚‚ + 6 Hβ‚‚O ⚑

πŸ”΄ Question 24:
Describe glycolysis with schematic flow.
🟒 Answer:
Site: Cytoplasm.
Stages:
 1️⃣ Phosphorylation: Glucose β†’ Glucose-6-P β†’ Fructose-1,6-bisP.
 2️⃣ Cleavage: Splits into 2 triose phosphates.
 3️⃣ Oxidation: Forms pyruvate, 4 ATP (gross), 2 ATP (net), 2 NADH.
Equation:
 Glucose + 2 NAD⁺ + 2 ADP + 2 Pi β†’ 2 Pyruvate + 2 NADH + 2 ATP + 2 Hβ‚‚O.
πŸ’‘ Result: Energy captured in ATP and NADH for next steps.

πŸ”΄ Question 25:
Explain the Krebs cycle with major products and significance.
🟒 Answer:
Site: Mitochondrial matrix.
Steps:
 1️⃣ Acetyl CoA + OAA β†’ Citrate.
 2️⃣ Series of oxidation & decarboxylation reactions.
 3️⃣ Regeneration of OAA.
Products (per turn): 3 NADH, 1 FADHβ‚‚, 1 ATP, 2 COβ‚‚.
Significance:
β€ƒβœ”οΈ Major source of NADH & FADHβ‚‚.
β€ƒβœ”οΈ Connects anabolic & catabolic pathways.
β€ƒβœ”οΈ Provides intermediates for biosynthesis.

πŸ”΄ Question 26:
Explain the mechanism of electron transport system (ETS) and oxidative phosphorylation.
🟒 Answer:
Location: Inner mitochondrial membrane.
Carriers: NADH β†’ FMN β†’ CoQ β†’ Cyt b β†’ Cyt c β†’ Cyt a/a₃ β†’ Oβ‚‚.
Process:
 1️⃣ Electrons flow β†’ energy released pumps H⁺ into intermembrane space.
 2️⃣ Gradient drives ATP synthase β†’ ATP formed (chemiosmosis).
 3️⃣ Oβ‚‚ accepts electrons β†’ Hβ‚‚O.
Result: ~34 ATP generated.
βœ… This is oxidative phosphorylation. ⚑

πŸ”΄ Question 27:
Differentiate between aerobic respiration, anaerobic respiration, and fermentation.
🟒 Answer:
Feature Aerobic Anaerobic Fermentation
Oβ‚‚ requirement Present Absent Absent
Site Mitochondria Cytoplasm Cytoplasm
End products COβ‚‚ + Hβ‚‚O Lactic acid Alcohol + COβ‚‚
ATP yield 36 2 2
Organisms Higher plants Muscle cells Yeast 🍞
πŸ’‘ Fermentation is a type of anaerobic respiration.

πŸ”΄ Question 28:
Explain respiratory quotient (RQ) and write its values for different substrates.
🟒 Answer:
Definition: Ratio of COβ‚‚ evolved to Oβ‚‚ consumed.
 RQ = COβ‚‚ / Oβ‚‚
Substrate Equation RQ
Carbohydrate C₆H₁₂O₆ + 6Oβ‚‚ β†’ 6COβ‚‚ + 6Hβ‚‚O 1
Fat 2C₅₇H₁₁₀O₆ + 163Oβ‚‚ β†’ 114COβ‚‚ + 110Hβ‚‚O <1 Protein Variable <1 Organic acid Cβ‚„H₆Oβ‚… + 3Oβ‚‚ β†’ 4COβ‚‚ + 3Hβ‚‚O >1
πŸ’‘ RQ indicates type of substrate used.

πŸ”΄ Question 29:
Discuss amphibolic nature of respiration.
🟒 Answer:
Definition: Acts as both catabolic (breakdown) and anabolic (synthesis) pathway.
Catabolic: Glucose oxidized to COβ‚‚ + Hβ‚‚O.
Anabolic: Intermediates used to synthesize amino acids, fats, nucleotides.
πŸ’‘ Hence, respiration is amphibolic β€” center of metabolism.

πŸ”΄ Question 30:
Write short notes on fermentation and its economic importance.
🟒 Answer:
Fermentation: Incomplete oxidation of glucose in absence of Oβ‚‚ by microbes.
 Glucose β†’ Alcohol + COβ‚‚ + 2 ATP.
Types:
 1️⃣ Alcoholic (Yeast) 🍢
 2️⃣ Lactic acid (Bacteria) πŸ§€
Uses:
β€ƒβœ”οΈ Brewing and baking industries.
β€ƒβœ”οΈ Dairy products (curd, cheese).
β€ƒβœ”οΈ Production of biofuels.

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